ephrin type a receptor 5  (Santa Cruz Biotechnology)

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: A The heatmaps and volcano plot show that EPHA5 expression was upregulated in FTC tissues. B Box plot analysis of EPHA5 expression differences. The data are sourced from the datasets of GEO ( https://www.ncbi.nlm.nih.gov/geo/ ): GSE27155. C qPCR analysis of EPHA5 mRNA between Nthy-ori-3-1 and FTC cells, * p < 0.05. n = 6. D Western blot analysis of EPHA5 protein expression in FTC-133 and FTC-238 cells. * p < 0.05. n = 3. E Representative images from immunohistochemically staining of EPHA5 in FTC patient tissues and matching paraneoplastic tissues (EPHA5 is localized in the cell membrane and endoplasmic reticulum). scale bars, 50 μm. n = 20.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques: Expressing, Western Blot, Staining, Membrane

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: A Western blot analysis of EPHA5, PCNA, Cyclin D1, Caspase-3, Cleaved-caspase-3, and β -actin in FTC-133 and FTC-238 cells. ** p < 0.01, n = 3. B , C CCK-8 assay analysis of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. ** p < 0.01, n = 6. D Representative TUNEL staining of FTC cells; the nuclei were stained with DAPI. Scale bar, 200 μm. TUNEL scores. The relative number of apoptotic cells is represented as TUNEL-positive cells/DAPI. ** p < 0.01, n = 6. E Colony formation assay of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. ** p < 0.01, n = 6.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques: Western Blot, CCK-8 Assay, Transfection, TUNEL Assay, Staining, Colony Assay

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: A Interaction network with EPHA5, from Biogrid database. B Co-immunoprecipitation experiments were conducted on FTC-238 cells lysates with anti-EPHA5 and anti-STAT3, and then the precipitation was subsequently analyzed by western blotting with anti-STAT3 and anti-EPHA5. C Immunofluorescence analyses were performed using anti-EPHA5 antibodies or anti-STAT3 antibodies. Representative images are shown. Scale bar, 25 μm. D Western blot analysis of STAT3, p-STAT3, β -actin in FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. ** p < 0.01. n = 3. E QRT-PCR analysis of BCL-XL, Myc, Timp-1, and CyclinD1 mRNA in FTC-133 and FTC-238 cells with EPHA5 knockdown. * p < 0.05; ** p < 0.01, n = 8.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques: Immunoprecipitation, Western Blot, Immunofluorescence, Transfection, Quantitative RT-PCR, Knockdown

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: A Western blot analysis of PCNA, p-STAT3, STAT3, Caspase-3, Cleaved-caspase3 and β -actin in FTC-133 and FTC-238 cells. * p < 0.05; ** p < 0.01, n = 3. B Colony formation assay of FTC-133 and FTC-238 cell. ** p < 0.01, n = 6. C Representative TUNEL staining of FTC cells; the nuclei were stained with DAPI. Scale bar = 200 μm. TUNEL scores. The relative number of apoptotic cells is represented as TUNEL-positive cells/DAPI. ** p < 0.01, n = 6. D Western blot analysis of STAT3, cleaved-caspase-3, and β-actin in FTC-133 and FTC-238 cells. ** p < 0.01, n = 3. E Colony formation assay of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 or si-EPHA5 + si-STAT3 respectively. * p < 0.05, ** p < 0.01, n = 3. F Representative FITC and PI staining of FTC cells. Scale bar, 200 μm. The relative number of apoptotic cells is represented as FITC-positive cells ratio. ** p < 0.01, n = 3.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques: Western Blot, Colony Assay, TUNEL Assay, Staining, Transfection

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: A Western blot analysis of p-STAT3, STAT3, PCNA, Caspase-3, Cleaved-caspase-3, β -actin in FTC-133 and FTC-238 cells. ** p < 0.01, ## p < 0.01, n = 3. B Colony formation assay of FTC-133 and FTC-238 cells. ** p < 0.01, ## p < 0.01, n = 6. C Representative TUNEL staining of FTC cells; the nuclei were stained with DAPI. Scale bar = 200 μm. TUNEL scores. The relative number of apoptotic cells is represented as TUNEL-positive cells/DAPI. * p < 0.05, ## p < 0.01, n = 6. D Western blot analysis of STAT3, cleaved-caspase-3, and β-actin in FTC-133 and FTC-238 cells. ** p < 0.01, n = 3. E Colony formation assay of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 or si-EPHA5 + si-STAT3 respectively. ** p < 0.01, n = 3. F Representative FITC and PI staining of FTC cells. Scale bar, 200 μm. The relative number of apoptotic cells is represented as FITC-positive cells ratio. * p < 0.05, n = 3. G Images of the xenograft tumors formed in nude mice subcutaneously injected with FTC-238 cells with EPHA5 knockdown or overexpression treated with SH-4-54. H , I Tumor weight was measured after the tumors were removed. Tumor growth curves were measured after injection of FTC238 cells with EPHA5 knockdown or EPHA5 overexpression treated with SH-4-54. * p < 0.05, ## p < 0.01, n = 5.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques: Western Blot, Colony Assay, TUNEL Assay, Staining, Transfection, Injection, Knockdown, Over Expression

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques:

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: A The heatmaps and volcano plot show that EPHA5 expression was upregulated in FTC tissues. B Box plot analysis of EPHA5 expression differences. The data are sourced from the datasets of GEO ( https://www.ncbi.nlm.nih.gov/geo/ ): GSE27155. C qPCR analysis of EPHA5 mRNA between Nthy-ori-3-1 and FTC cells, * p < 0.05. n = 6. D Western blot analysis of EPHA5 protein expression in FTC-133 and FTC-238 cells. * p < 0.05. n = 3. E Representative images from immunohistochemically staining of EPHA5 in FTC patient tissues and matching paraneoplastic tissues (EPHA5 is localized in the cell membrane and endoplasmic reticulum). scale bars, 50 μm. n = 20.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques: Expressing, Western Blot, Staining, Membrane

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: A Western blot analysis of EPHA5, PCNA, Cyclin D1, Caspase-3, Cleaved-caspase-3, and β -actin in FTC-133 and FTC-238 cells. ** p < 0.01, n = 3. B , C CCK-8 assay analysis of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. ** p < 0.01, n = 6. D Representative TUNEL staining of FTC cells; the nuclei were stained with DAPI. Scale bar, 200 μm. TUNEL scores. The relative number of apoptotic cells is represented as TUNEL-positive cells/DAPI. ** p < 0.01, n = 6. E Colony formation assay of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. ** p < 0.01, n = 6.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques: Western Blot, CCK-8 Assay, Transfection, TUNEL Assay, Staining, Colony Assay

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: A Interaction network with EPHA5, from Biogrid database. B Co-immunoprecipitation experiments were conducted on FTC-238 cells lysates with anti-EPHA5 and anti-STAT3, and then the precipitation was subsequently analyzed by western blotting with anti-STAT3 and anti-EPHA5. C Immunofluorescence analyses were performed using anti-EPHA5 antibodies or anti-STAT3 antibodies. Representative images are shown. Scale bar, 25 μm. D Western blot analysis of STAT3, p-STAT3, β -actin in FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. ** p < 0.01. n = 3. E QRT-PCR analysis of BCL-XL, Myc, Timp-1, and CyclinD1 mRNA in FTC-133 and FTC-238 cells with EPHA5 knockdown. * p < 0.05; ** p < 0.01, n = 8.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques: Immunoprecipitation, Western Blot, Immunofluorescence, Transfection, Quantitative RT-PCR, Knockdown

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: A Western blot analysis of PCNA, p-STAT3, STAT3, Caspase-3, Cleaved-caspase3 and β -actin in FTC-133 and FTC-238 cells. * p < 0.05; ** p < 0.01, n = 3. B Colony formation assay of FTC-133 and FTC-238 cell. ** p < 0.01, n = 6. C Representative TUNEL staining of FTC cells; the nuclei were stained with DAPI. Scale bar = 200 μm. TUNEL scores. The relative number of apoptotic cells is represented as TUNEL-positive cells/DAPI. ** p < 0.01, n = 6. D Western blot analysis of STAT3, cleaved-caspase-3, and β-actin in FTC-133 and FTC-238 cells. ** p < 0.01, n = 3. E Colony formation assay of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 or si-EPHA5 + si-STAT3 respectively. * p < 0.05, ** p < 0.01, n = 3. F Representative FITC and PI staining of FTC cells. Scale bar, 200 μm. The relative number of apoptotic cells is represented as FITC-positive cells ratio. ** p < 0.01, n = 3.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques: Western Blot, Colony Assay, TUNEL Assay, Staining, Transfection

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: A Western blot analysis of p-STAT3, STAT3, PCNA, Caspase-3, Cleaved-caspase-3, β -actin in FTC-133 and FTC-238 cells. ** p < 0.01, ## p < 0.01, n = 3. B Colony formation assay of FTC-133 and FTC-238 cells. ** p < 0.01, ## p < 0.01, n = 6. C Representative TUNEL staining of FTC cells; the nuclei were stained with DAPI. Scale bar = 200 μm. TUNEL scores. The relative number of apoptotic cells is represented as TUNEL-positive cells/DAPI. * p < 0.05, ## p < 0.01, n = 6. D Western blot analysis of STAT3, cleaved-caspase-3, and β-actin in FTC-133 and FTC-238 cells. ** p < 0.01, n = 3. E Colony formation assay of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 or si-EPHA5 + si-STAT3 respectively. ** p < 0.01, n = 3. F Representative FITC and PI staining of FTC cells. Scale bar, 200 μm. The relative number of apoptotic cells is represented as FITC-positive cells ratio. * p < 0.05, n = 3. G Images of the xenograft tumors formed in nude mice subcutaneously injected with FTC-238 cells with EPHA5 knockdown or overexpression treated with SH-4-54. H , I Tumor weight was measured after the tumors were removed. Tumor growth curves were measured after injection of FTC238 cells with EPHA5 knockdown or EPHA5 overexpression treated with SH-4-54. * p < 0.05, ## p < 0.01, n = 5.

Article Snippet: Reagents used for western blot analysis included primary antibodies against EPHA5 (obtained from Santa Cruz, USA), STAT3 (purchased from Santa Cruz, USA), p-STAT3 (sourced from Santa Cruz, USA), cleaved caspase-3 (acquired from Beyotime Institute of Biotechnology, Shanghai, China), PCNA (obtained from Proteintech, Wuhan, China), Cyclin D1 (sourced from Proteintech, Wuhan, China) and β -actin (acquired from Beyotime Institute of Biotechnology, Shanghai, China).

Techniques: Western Blot, Colony Assay, TUNEL Assay, Staining, Transfection, Injection, Knockdown, Over Expression

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway.

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: Fig. 1 High expression of EPHA5 was correlated with poor prognosis of thyroid carcinoma patients. A The heatmaps and volcano plot show that EPHA5 expression was upregulated in FTC tissues. B Box plot analysis of EPHA5 expression differences. The data are sourced from the datasets of GEO (https://www.ncbi.nlm.nih.gov/geo/): GSE27155. C qPCR analysis of EPHA5 mRNA between Nthy-ori-3-1 and FTC cells, *p < 0.05. n = 6. D Western blot analysis of EPHA5 protein expression in FTC-133 and FTC-238 cells. *p < 0.05. n = 3. E Representative images from immunohistochemically staining of EPHA5 in FTC patient tissues and matching paraneoplastic tissues (EPHA5 is localized in the cell membrane and endoplasmic reticulum). scale bars, 50 μm. n = 20.

Article Snippet: After that, the cells were incubated with 5% BSA for half an hour at room temperature and then exposed to primary antibodies against EPHA5 (1:200, Santa Cruz, USA) and STAT3 (1:500, Proteintech, Wuhan, China) overnight at 4 °C.

Techniques: Expressing, Western Blot, Staining, Membrane

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway.

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: Fig. 2 EPHA5 promote proliferation and inhibit apoptosis in FTC cells. A Western blot analysis of EPHA5, PCNA, Cyclin D1, Caspase-3, Cleaved-caspase-3, and β-actin in FTC-133 and FTC-238 cells. **p < 0.01, n = 3. B, C CCK-8 assay analysis of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. **p < 0.01, n = 6. D Representative TUNEL staining of FTC cells; the nuclei were stained with DAPI. Scale bar, 200 μm. TUNEL scores. The relative number of apoptotic cells is represented as TUNEL-positive cells/DAPI. **p < 0.01, n = 6. E Colony formation assay of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. **p < 0.01, n = 6.

Article Snippet: After that, the cells were incubated with 5% BSA for half an hour at room temperature and then exposed to primary antibodies against EPHA5 (1:200, Santa Cruz, USA) and STAT3 (1:500, Proteintech, Wuhan, China) overnight at 4 °C.

Techniques: Western Blot, CCK-8 Assay, Transfection, TUNEL Assay, Staining, Colony Assay

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway.

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: Fig. 3 The interaction of EPHA5 with STAT3 and the expressions of STAT3-mediated transcription downstream targets. A Interaction network with EPHA5, from Biogrid database. B Co-immunoprecipitation experiments were conducted on FTC-238 cells lysates with anti- EPHA5 and anti-STAT3, and then the precipitation was subsequently analyzed by western blotting with anti-STAT3 and anti-EPHA5. C Immunofluorescence analyses were performed using anti-EPHA5 antibodies or anti-STAT3 antibodies. Representative images are shown. Scale bar, 25 μm. D Western blot analysis of STAT3, p-STAT3, β-actin in FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. **p < 0.01. n = 3. E QRT-PCR analysis of BCL-XL, Myc, Timp-1, and CyclinD1 mRNA in FTC-133 and FTC-238 cells with EPHA5 knockdown. *p < 0.05; **p < 0.01, n = 8.

Article Snippet: After that, the cells were incubated with 5% BSA for half an hour at room temperature and then exposed to primary antibodies against EPHA5 (1:200, Santa Cruz, USA) and STAT3 (1:500, Proteintech, Wuhan, China) overnight at 4 °C.

Techniques: Immunoprecipitation, Western Blot, Transfection, Quantitative RT-PCR, Knockdown

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway.

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: Fig. 4 P-STAT3 inhibition inhibited proliferation and enhanced apoptosis of FTC cells with EPHA5 knockdown. A Western blot analysis of PCNA, p-STAT3, STAT3, Caspase-3, Cleaved-caspase3 and β-actin in FTC-133 and FTC-238 cells. *p < 0.05; **p < 0.01, n = 3. B Colony formation assay of FTC-133 and FTC-238 cell. **p < 0.01, n = 6. C Representative TUNEL staining of FTC cells; the nuclei were stained with DAPI. Scale bar = 200 μm. TUNEL scores. The relative number of apoptotic cells is represented as TUNEL-positive cells/DAPI. **p < 0.01, n = 6. D Western blot analysis of STAT3, cleaved-caspase-3, and β-actin in FTC-133 and FTC-238 cells. **p < 0.01, n = 3. E Colony formation assay of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 or si-EPHA5 + si-STAT3 respectively. *p < 0.05, **p < 0.01, n = 3. F Representative FITC and PI staining of FTC cells. Scale bar, 200 μm. The relative number of apoptotic cells is represented as FITC-positive cells ratio. **p < 0.01, n = 3.

Article Snippet: After that, the cells were incubated with 5% BSA for half an hour at room temperature and then exposed to primary antibodies against EPHA5 (1:200, Santa Cruz, USA) and STAT3 (1:500, Proteintech, Wuhan, China) overnight at 4 °C.

Techniques: Inhibition, Knockdown, Western Blot, Colony Assay, TUNEL Assay, Staining, Transfection

Journal: Oncogenesis

Article Title: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway.

doi: 10.1038/s41389-025-00556-3

Figure Lengend Snippet: Fig. 6 EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway.

Article Snippet: After that, the cells were incubated with 5% BSA for half an hour at room temperature and then exposed to primary antibodies against EPHA5 (1:200, Santa Cruz, USA) and STAT3 (1:500, Proteintech, Wuhan, China) overnight at 4 °C.

Techniques: